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Albert Stephen Lossinsky, Ph.D.
Area of Specialty: Research Scientist, Cell Culture Neuroanatomy and Neuropathology.
Professional Appointment: Senior Research Scientist, New Jersey Neuroscience Institute, JFK Medical Center, Edison,
New Jersey .
Academic Appointment: Professor of Neuroscience, Seton Hall
University School of Graduate Medical Education, South Orange, New Jersey .
Graduate Education: 1974. Master of Science. Emporia State University, Emporia, Kansas. Microbiology and Electron Microscopy. 1994. Doctor of Science. Polish Academy of Sciences, Medical Research Center, Warsaw, Poland. Neurobiology and Neuropathology.
Honors and Awards: Outstanding Young Men of America, Baltimore, MD, 1974; Kansas Wesleyan University Alumni Career Achievement Award, 1994.
Research Interests: Vascular pathology and blood-brain barrier (BBB) structure and function. Understanding the morphologic and ultrastructural nature of adhesion and transendothelial cell transport of leukocytes, tumor cells and pathogenic microorganisms (bacteria, yeasts, viruses) across the injured mammalian BBB using in vivo and in vitro models. To develop an in vitro model of the neurovascular unit and models of BBB pathogenesis that include neuronal and glial cell populations. Understanding endothelial cell cytoskeletal dynamics of leukocyte transmigration across the injured BBB during inflammatory conditions. Using enzyme cytochemical markers to study vascular and neuronal injury with light and electron microscopic techniques. Basic research on biofilms in the CNS related to the tissue response to implanted foreign objects including microelectrodes and the development of neuroprosthetic devices. Application of immunocytochemical techniques using morphologic and quantitative approaches.
Professional Memberships: AAAS, American Society for Cell Biology; American Society of Microbiologists; FASEB; Society for Neuropathologists; North American Vascular Biology Organization; Microscopy Society of America; Society for Neuroscience.
Co-Managing Editor, Co-Chairperson of a BBB Internet Website: www.bloodbrainbarrier.org. This website is being established in conjunction with Muhammad Mukhtar, Ph.D., Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA. The purpose of this society will be to organize teaching and research seminars on topics related to blood-brain barrier in human medicine.
History of Extramural Grant Awards/Grants Pending (9/2007):
Source: National Institutes of Neurological Diseases and Stroke (NINDS)
R01 NS18079; Ultracytochemistry of Transport Mechanisms in Altered BBB
Dates Active: 12/1/81 - 11/30/84
Total Direct Costs: $ 23,100
Albert S. Lossinsky, M.S., P.I
Source: NINDS R01 NS17271
Microvasculature in Normal and Pathological Brain
Dates: 4/1/81 - 3/31/85
Total direct costs: $95,298
Andrzej W. Vorbrodt, M.D., P.I.
Albert S. Lossinsky, M.S., Co-investigator
Source: NINDS 2R01 NS17271-04-09
Microvasculature in Normal and Pathological Brain
Dates: 9/30/84 - 11/30/89
Total direct costs: $197,562
Andrzej W. Vorbrodt, M.D., P.I.
Albert S. Lossinsky, M.S., Co-investigator
Source: Basic Research Study Grant (BRSG - IBR)
High-voltage Electron Microscopic Studies in
BBB Injury
Dates 4/1/89 - 3/31/90
Total direct costs: $2,000
Albert S. Lossinsky, M.S., P.I.
Source: National Institutes of Aging (NIA) RO1 AG10279-01
Transport of Modified Albumin Across BBB
Total direct costs: $497,000
Dates: 5-1-92 - 4-30-97
Andrzej W. Vorbrodt, M.D., P.I.
Albert S. Lossinsky, M.S., Co-Investigator
Source: National Institutes of Health (NIAID).
Mechanisms of HIV Neuroinvasion.
Albert S. Lossinsky. Ph.D., P.I. of Subcontract to conduct ultrastructural and immunoultrastructural studies for Milan Fiala. M.D., P.I. of contract, Department of Medicine, UCLA School of Medicine, Los Angeles , CA .
Total direct costs: $22,000/year.
Source: National Institutes of Health (NIAID), RO3 AI0556536-01
Leukocyte Transmigration in Neonatal Candida Meningitis.
Transport of Modified Albumin Across Blood-Brain Barrier
Total direct costs: $150,000
Dates: 7/1/03 - 6/30/05
Albert S. Lossinsky, Ph.D., P.I.
Source: National Institutes of Health (NIAID), 1 R21AI076866-01
Candida albi cans Meningitis in Immunosuppressed and Adult Rats.
Submitted February 1, 2007 to the NIAID (PTHE) Study Section.
Total direct costs: $200,000.Albert S. Lossinsky, Ph.D., P.I.
Source: National Institutes of Health (NINDS), 1 R21-----
Rat Brain Inflammation after Electrical Stimulation.
Submitted June, 2007 to the NIH.
Total direct costs: $200,000.
Albert S. Lossinsky, Ph.D., P.I.
Ad Hoc Reviewer for Scientific Journals: Acta Neuropathologica, American J Pathology, Brain Research, Current HIV Research, J Comparative Neurology, J Neuroimmunology, J Neuropathology & Experimental Neurology, Microvascular Research, Neurodegeneration, Stroke.
Other Interests: Walking, jogging, fly fishing, fly tying, gardening, classical music, reading and travel.
Selected Publications:
1. Garcia, J.H., Lossinsky A.S. , Kauffman, F.C. and Conger, K.A.: Neuronal ischemic injury: Light microscopy, ultrastructure and biochemistry. Acta Neuropathol. (Berl.) 43:85-95, 1978.
2. Conger, K.A., Garcia, J.H., Lossinsky, A.S. and Kauffman, F.C.: The effect of aldehyde fixation on selected substrates for energy metabolism and amino acids in mouse brain. J. Histochem. and Cytochem. 26:423-433, 1978.
3. Lossinsky, A.S. , Garcia, J.H., Iwanowski, L. and Lightfoote, W.E., Jr.: New Ultrastructural Evidence for a protein transport system in endothelial cells of Gerbil brains. Acta Neuropathol. (Berl.) 47:105-110, 1979.
4. Garcia, J.H., Klatzo, I. , Archer, T., and Lossinsky, A.S. : Arterial air embolism: structural effects on the gerbil brain. Stroke 12(4):414-421, 1981.
5. Vorbrodt, A.W., Lossinsky, A.S. and Wisniewski, H.M.: Enzyme cytochemistry of blood-brain barrier disturbances. Acta Neuropathologica (Berl) IN: Cerebrovascular Transport Mechanisms. Hossmann, K.-A., and Klatzo, I. (eds) Berlin-Heidelberg, Springer Verlag, Suppl VII: 43-57, 1983.
6. Lossinsky, A.S. , Vorbrodt, A.W. and Wisniewski, H.M.: Characterization of endothelial cell transport in the developing mouse blood-brain barrier. Dev. Neurosci. 8:61-75, 1986.
7. Vorbrodt, A.W., Lossinsky, A.S. , and Wisniewski, H.M.: Localization of alkaline phosphatase activity in endothelia of developing and mature mouse blood-brain barrier. Dev. Neurosci.8:1-13,1986.
8. Lossinsky, A.S. , Moretz, R.C., Carp, R.I., and Wisniewski, H.M.: Ultrastructural observations of spinal cord lesions and blood-brain barrier changes in scrapie infected mice. Acta Neuropathol. (Berl.) 73:43-52, 1987.
8. Vorbrodt, A.W., Dobrogowska, D.H., Kim Y.S., Lossinsky, A.S. , and Wisniewski, H.M.: Ultrastructural studies of glycoconjugates in brain micro-blood vessels and amyloid plaques in scrapie-infected mice. Acta Neuropathol. (Berl.) 75:277-287, 1988.
9. Lossinsky, A.S. , Song, M.J., and Wisniewski, H.M.: High-voltage electron microscopic studies of endothelial tubular structures in mouse blood-brain barrier following brain trauma. Acta Neuropathol. 77:480-488, 1989.
10. Lossinsky, A.S. , Badmajew, V., Robson, J., Moretz, R.C., and Wisniewski, H.M.: Sites of egress of inflammatory cells and horseradish peroxidase transport in a murine model of chronic relapsing experimental allergic encephalomyelitis. Acta Neuropathol. 78:359-371, 1989.
11. Lossinsky, A.S. , Song, M.J., Pluta, R., Moretz, R.M., and Wisniewski, H.M.: Combined conventional transmission, scanning and high-voltage electron microscopy of the same blood vessel for the study of blood-brain barrier inflammation. Microvasc. Res. 40:427-438, 1990.
12. Wisniewski, H.M., Vorbrodt, A.W., Wegiel, J., Morys, J., and Lossinsky, A.S. : Ultrastructure of the cells forming amyloid fibers in Alzheimer's disease and scrapie. Am J Med Gen Vol 37: 287-297, 1990.
13. Wisniewski, H.M., and Lossinsky, A.S. : Structural and functional aspects of the interaction of inflammatory cells with the blood-brain barrier in experimental brain inflammation. Brain Pathol, (mini-review) 1:89-96, 1991.
14. Lossinsky, A.S. , Pluta, R., Song, M.J., Badmajew, V., Moretz, R.C., and Wisniewski, H.M.: scanning and high-voltage electron microscopic study of the injured mouse blood-brain barrier. Microvasc. Res. 41:299-310, 1991.
15. Pluta, R., Lossinsky, A.S. , Mossakowski, M.J., Faso, L., and Wisniewski, H.M.: Reassessment of a new model of complete cerebral ischemia in rats. Method of induction of clinical death, pathophysiology and cerebrovascular pathology. Acta Neuropathol. 83:1-11, 1991.
16. Pluta, R., Lossinsky, A.S. , Wisniewski, H.M., and Mossakowski, M.J.: Early blood-brain barrier changes in the rat following transient complete cerebral ischemia induced by cardiac arrest. Brain Res. 633:41-52, 1994.
17. Pluta, R., Kida, E., Lossinsky, A.S. , Golabek, A.A., Mossakowski, M.J., and Wisniewski, H.M.: Complete cerebral ischemia with short-term survival in rats induced by cardiac arrest. I. Extracellular accumulation of Alzheimer's ß-amyloid protein precursor in the brain. Brain Res. 649:323-328, 1994.
18. Kida, E., Pluta, R., Lossinsky, A.S. , Golabek, A.A, Choi-Miura, –H., Wisniewski, H.M., and M.J. Mossakowski: Complete cerebral ischemia with short-term survival in rat induced by cardiac arrest. II. Extracellular and intracellular accumulation of apolipoproteins E and J in the brain. Brain Res. 674:341-346, 1995.
19. Lossinsky, A.S. , Vorbrodt, A.W. and Wisniewski, H.M.: Scanning and transmission electron microscopic studies of microvascular pathology in the osmotically impaired blood-brain barrier. J. Neurocytol. 24:795-806, 1995.
20. Lossinsky, A.S. , Mossakowski, M.J., Pluta, R., and Wisniewski, H.M.: Intercellular adhesion molecule-1 (ICAM-I) upregulation in human brain tumors as an expression of increased blood-brain barrier permeability. Brain Pathol. 5:339-344, 1995.
21. Lossinsky, A.S. , Wisniewski, H.M., Dambska, M. and Mossakowski, M.J.: Ultrastructural studies of PECAM-1/CD31 expression in the developing mouse blood-brain barrier with the application of a pre-embedding technique. Folia Neuropathol. 35:163-169, 1997.
22. Dobrogowska, D.H, Lossinsky, A.S. , Tarnawski M. and Vorbrodt, A.W.: Increased blood-brain barrier permeability and endothelial abnormalities induced by vascular endothelial cell growth factor. J Neurocytol 27:163-173, 1998.
23. Lossinsky, A.S. , Wisniewski H.M.: Immunoultrastructural expression of ICAM-1 and PECAM-1 occurs prior to structural maturity of the murine blood-brain barrier. Develop Neurosci 20:518-524, 1998.
24. Lossinsky, A.S. , Buttle, K.F., Pluta, R., Wisniewski, H.M. and Mossakowski, M.J.: Immunoultrastructural expression of intercellular adhesion molecule-1 (ICAM-1/CD54) in endothelial cell vesiculo-tubular structures and vesiculo-vacuolar organelles (VVO) in blood-brain barrier development and injury. Cell Tiss Res 295:77-88, 1999.
25. Liu, N.Q., Lossinsky, A.S. , Popik W, Li, X., Gujuluva, C., Kriederman, B., Roberts. J., Pushkarsky, T., Bukrinsky, M., Witte, M., Weinand, M. and Fiala, M: Human immunodeficiency virus type 1 enters brain microvascular endothelia by macropinocytosis dependent on lipid rafts and mitogen-activated protein kinase signaling pathway. J Virol 76(10):6689-6700, 2002.
26. Lossinsky, A.S. and Shivers, R.R. Studies of Cerebral Endothelium by Scanning and High-Voltage Electron Microscopy. The Blood-Brain Barrier: Biology and Research Protocols. Ed. Nag. S. Human Press, INC., Methods Mol Med 89: 67-82, 2003.
27. Lossinsky, A.S. and Shivers, R.R.: Structural Pathways for Macromolecular and Cellular Transport Across the Blood-Brain Barrier during Inflammatory Conditions. A Review. Histol & Histopathol, 19:535-564, 2004.
28. McCreery, D., Pikov, V. Lossinsky A.S. , Bullara L. and Agnew W.: Arrays for functional microstimulation of the lumbosacral spinal cord. IEEE Trans. on Neural Systems & Rehabilitation Engineering 12:195-207, 2004.
29. Fiala, M., Eshleman A.J., Cashman J., Lin J., Lossinsky, A.S. , Suarez, V., Yang W., Zang J., Popik W., Singer E., Chiappelli F., Carro E., Weinand M., Witte, M., and Arthos J.: Cocaine increases human immunodeficiency virus type 1 neuroinvasion through remodeling brain microvascular endothelial cells. J Neurovirol. 11:281-291, 2005.
30. Fiala M., Lin J., Ringman J., Kermani-Arabv V., Tsao G., Patel A., Lossinsky, A.S. , Graves M.C., Gustavson A., Sayre J., Sofroni E., Suarez T., Chiappelli F. and Bernard G.: Ineffective phagocytosis of amyloid-beta by macrophages of Alzheimer's disease patients. J Alzheimer's Dis. 7:221-232; discussion 255-262, 2005.
31. Lossinsky, A.S., Jong, A., Fiala, M. Mukhtar, M., Buttle K.F., Ingram M. L.: The histopathology of Candida albicans invasion in neonatal rat tissues and in the human blood-brain barrier in culture revealed by light, scanning, transmission and immunoelectron microscopy. Histol and Histopathol. 21:1029-1041, 2006.
32. Kamai, Y., Chiang, L.Y., Lopez Bezerra, L.M., Doedt, T., Lossinsky, A.S., Sheppard , D.C. , Filler, S.G.: Interactions of Aspergillus fumigatus with vascular endothelial cells. Med Mycol 44 Suppl:115-117, 2006.
33. Acheampong, E., Parveen, Z., Mengisty, A., Ngoubilly, N., Wigdahl, B., Lossinsky, A.S., Pomerantz, R.J., Mukhtar, M. The cholesterol depleting drugs statin protect post-mitotically differentiated human neurons against ethanol and HIV-1-induced oxidative stress in vitro. J. Virol. 81:1492-1501, 2007.